Regulatory

Part:BBa_K1724000:Design

Designed by: Zhanru Chen   Group: iGEM15_SCUT   (2015-09-05)


Pcada


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

FP:GTTTCTTCGAATTCGCGGCCGCTTCTAGATTGACTCTGTAGTTGCTACA

RP:GTTTCTTCCTGCAGCGGCCGCTACTAGT ATTGCACACC CTGTAGCAAC TACAGA


With 4 standard restriction enzyme sites.

Source

OVERLAP PCR

References